From DNA to Therapeutic Protein in CHO Cells
How did CHO cells become the most important cells in biotechnology?
In 1957, a sample of cells from the ovary of a Chinese hamster was cultured and described. Unknowingly, the scientists establishing the Chinese Hamster Ovary (CHO) cells had kicked off a revolution within the field of biotech and biosynthesis of therapeutic proteins.
Today, most of the therapeutic proteins on the market are produced in large scale bioreactors using CHO cells derived from that same cell line from 1957.
Although cell culture methods and resulting yields have improved tremendously since the first recombinant proteins were made from CHO cells in 1978, we continue to see breakthroughs today.
One such breakthrough was contributed by ExcellGene scientists.
The orbital shaking of small bioreactors for high throughput of cell cultures was invented by Maria Wurm, a technology which is now industry standard and led to significant increases in yield due to the insights it provided.
The Full Talk: From DNA to Therapeutic Protein in CHO Cells - Plasmids, Cells, Process, Bioreactors to 10 g/L or More - History, Cytogenetics, Population Genetics, Scale-down and Scale-up, Regulatory and Manufacturability
Topics brought up include: