Knowledge base

Welcome to the lab.

Learn more about the discoveries that paved the way for ExcellGene’s scientific foundation. Videos, White Papers and publications, (co-) authored by F. M. Wurm, M.J. Wurm (De Jesus) and ExcellGene scientists over three decades.

Videos

  • From DNA to Therapeutic Protein in CHO cells

  • In 1957, a sample of cells from the ovary of a Chinese hamster was cultured and described. Unknowingly, the scientists establishing the Chinese Hamster Ovary (CHO) cells had kicked off a revolution within the field of biotech and biosynthesis of therapeutic proteins.

White papers

  • Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors Are High-Quality Antigens (Summary)

  • 2020 Paco Pino, Joeri Kint, Divor Kiseljak, Valentina Agnolon, Giampietro Corradin, Andrey V. Kajava, Paolo Rovero, Ronald Dijkman, Gerco den Hartog, Jason S. McLellan, Patrick O. Byrne, Maria J. Wurm and Florian M. Wurm Processes, 8(12), 1539; doi.org/10.3390/pr8121539

     

     

  • Cloning of CHO Cells, Productivity and Genetic Stability – a Discussion

     

  • 2017 Wurm, F.M., Wurm, M.J. Processes, 5, 20, doi:10.3390/pr5020020
    CHO History, CHO Evolution and CHO Genomics – an Unsolvable Enigma? 2014 Wurm, F.M. Hauser and Wagner: Animal Cell Biotechnology De Gruyter. Pp. 38-60

     

  • CHO Quasispecies – Implication for Manufacturing Processes

  • 2013 Wurm, F.M. Processes 1,3, 296-311
  • Commentary: Deciphering the Burgess Shale of Single-use Bioreactors 2010 Wurm, F.M. Next Generation Pharmaceutical

     

  • Medium and Process Development for High Yield, High-Density Suspension Cultures: from Low Throughput Spinner flasks to High Throughput Milliliter Reactors2009 De Jesus, M., Wurm, F.M. Bioprocess International, Vol 7, Suppl. 1, p.13-19

     

  • 25 Years of Recombinant Proteins from Reactor-Grown Cells: Where Do We Go From Here? 2009 Hacker, D.L., De Jesus, M., Wurm, F.M. Biotechnol Adv

     

  • Manufacturing of Biopharmaceuticals and Implications for Biosimilars 2007 Wurm, F. M. Kidney Blood Press Res. Vol. 30 Suppl 1, pp. 6-8.

     

  • Production of Recombinant Protein Therapeutics in Cultivated Mammalian Cells 2004 Wurm, F.M. Nature Biotechnology 22, 11, 1393-1398.

Publications

  • Naming CHO Cells for Bio-manufacturing: Genome Plasticity and Variant Phenotypes of Cell Populations in Bioreactors Question the Relevance of Old Names

     

  • 2021 Florian M. Wurm, Maria J. Wurm Biotechnology Journal, 2021, July, Volume 16, Issue 7; doi.org/10.1002/biot.202100165

     

  • Inducible Overexpression of the Mouse C-myc Protein in Mammalian Cells 1986 Wurm, F.M., .K.A. Gwinn, and R. E. Kingston Proc Natl Acad Sci USA : 83(15), pp. 5414-5418

     

     

  • Use of Fluorescence In Situ Hybridisation to Detect and Monitor Transfected and Amplified Sequences in Recombinant CHO Cells 1989 Pallavicini, M .G., DeTeresa, P.S. and Wurm, F.M. Dev. Biol. Stand., vol. 70, pp. 165-172

     

     

  • Designing Immunoadhesins for AIDS Therapy 1989 Capon, D.C., Chamow, S., Mordenti, J., Marsters, S.A., Gregory, T., Mitsuya, H., Byrn, R.A., Lucas, C. , Wurm, F.M., Groopman, J.E., Broder S. and Smith D.H. Nature 337 (6207) pp. 525-531

     

     

  • Review – Integration, Amplification and Stability of Plasmid Sequences in CHO Cell Cultures 1990 Wurm, F.M. Biologicals 18, pp.159-164.

     

  • Integration, Amplification and Stability of Plasmid Sequences in CHO Cell Cultures 1990 Wurm, F.M. Biologicals 18(3): 159-64

     

  • Presence and Transcription of Intracisternal A Particle Related Sequences in CHO Cells 1990 Anderson, K.P., Lie, Y.F., Low, M.L., Williams, S.R., Fennie, E.H., Nguyen, T.P. and Wurm, F.M. J. Virol, 64 (5), 2021-2032

     

  • Effects of Methotrexate (MTX) on Transfected DNA Stability in Mammalian Cells 1990 Pallavicini, M.G., DeTeresa, P.S., Rosette, C., Gray, J.W. and Wurm, F.M. Mol Cell Biol, 10 (1) pp. 401-404

     

  • High-Resolution Analysis of Functional Determinants on Human Tissue-Type Plasminogen-Activator 1991 Bennett, W.F., Paoni, N.F., Keyt, B.A., Botstein, D., Jones, A.J.S., Presta, L., Wurm, F.M., Zoller, M.J. J. Biol Chem 226 (8), pp.5191-5201

     

  • Characterisation of Recombinant CHO Cells 1994 Wurm, F.M. Cell and Tissue Culture: Laboratory Procedures, J.B. Griffiths, A. Doyle, D.G. Newell, Eds., Wiley Interscience

     

  • Plasmid Integration, Amplification and Cytogenetics in CHO Cells 1994 Wurm, F.M. and Petropoulos, C.P. Questions and comments. Biologicals 22, pp. 95-102

     

  • Gene Transfer and Amplification in CHO Cells – Efficient Methods for Maximising Specific Productivity and Assessment of Genetic Consequences 1996 Wurm, F.M., Johnson, A., Ryll, T., Köhne, C., Scherthan, H., Glaab, F., Lie, Y.S., Petropoulos C.J and Arathoon, W.R. Annals. N.Y. Acad. Sci. 782, pp 70-78

     

  • Transfecting Mammalian Cells: Optimisation of Critical Parameters Affecting Calcium-Phosphate Precipitate Formation 1996 Jordan, M., Schallhorn, A. and Wurm, F.M. Nucleic Acids Res 24(4), pp. 596-601

     

  • Aspects of Gene Transfer and Gene Amplification in Recombinant Mammalian Cells 1997 Wurm, F.M. Genetic Manipulation of Mammalian Cells. Hj. Hauser, R. Wagner, Eds. Walter de Gruyter, pp. 87-120

     

  • Principles of a Scaleable Transient Transfection and Expression Technology for Mammalian Cells 1997 Jordan, M., Köhne, C. and Wurm, F.M. M.J. Carrondo, B. Griffiths and J.P. Moreira: Animal Cell Technology – from Vaccines to Genetic Medicine, pp. 47-50. Kluwer Academic Publishers

     

  • Cytogenetic Characterisation of Recombinant Cells 1998 Wurm, F.M. and Schiffman, D. Methods in Molecular Biology – Mammalian Cell Biology. Nigel Jenkins, Ed. Humana press, pp. 49-59

     

  • Optimizing Production and Recovery of Immunoadhesins 1998 Wurm, F.M., Ashkenazi, A., Chamow, S. Antibody Fusion Proteins. S. Chamow and A. Ashkenazi, Eds. Wiley and Sons, pp. 281-308

     

  • Establishing and Developing CHO Cell Lines for Commercial Production of Recombinant Human Anti Rh D Antibodies 1999 De Jesus, M., Bourgeois, M., Jordan, M., Zahn, M., Mermod, N., Amstutz, H. and Wurm, F.M. Animal Cell Technology – Products from Cells, Cells as Products, A Bernard, B Griffiths, W Noe, F Wurm, Eds., Kluwer Acad. Publishers, Dordrecht, pp. 91-94

     

  • Establishing and Developing CHO Cell Lines for Commercial Production of Recombinant Human Anti Rh D Antibodies 1999 De Jesus, M., Bourgeois, M., Jordan, M., Zahn, M., Mermod, N., Amstutz, H. and Wurm, F.M. Animal Cell Technology – Products from Cells, Cells as Products, A Bernard, B Griffiths, W Noe, F Wurm, Eds., Kluwer Acad. Publishers, Dordrecht, pp. 91-94

     

  • Transient Gene Expression from Mammalian Cells – a New Chapter in Animal Cell Technology? 2001 Wurm, F.M. and Bernard, A. Cytotechnology 35, 155-156

     

  • Production of Recombinant Protein Therapeutics in Cultivated Mammalian Cells 2004 Wurm, F.M. Nature Biotechnology 22, 11, 1393-1398

     

  • Transfection of Adherent and Suspended Cells by Calcium Phosphate 2004 M. Jordan and F. Wurm Methods, Vol. 33, Nr. 2, pp. 136-43

     

  • Tubespin Satellites: a Fast Track Approach for Process Development with Animal Cells Using Shaking Technology 2004 De Jesus, M., Girard, P., Bourgeois, Baumgartner, M., Jacko, G., Amstutz, H. and Wurm, F.M. Biochemical Engineering Journal 17 (3), pp. 217-223

     

  • Orbital Shaker Technology for the Cultivation of Mammalian Cells in Suspension 2005 Muller, N., Girard, P., Jordan, M., Wurm, F.M. Biotechnol. Bioeng. 89 (4), pp. 400-406

     

  • Surface Wave Dynamics in Orbital Shaken Cylindrical Containers 2014 Reclari, M, Dreyer, M., Tissot, S., Obreschkow, D., Wurm, F.M., Farhat, M. Physics of Fluids 26, 052104, doi. 10.1063/1.4874612