OUR OFFERINGS
Expect great science.
It’s in our DNA.
ExcellGene’s reliable process and exceptional CHO cell hosts are the result of over twenty years of innovation and fine-tuning.
OUR SERVICES
A uniquely
science-driven CDMO
Since 2001, ExcellGene has developed proprietary technologies and know-how that are key for the delivery of its clients’ projects on a timely manner and with a high degree of customization.
CHO and HEK stable cell line development
Bioprocess Development
High Throughput Protein Production
Tox Material
cGMP Cell Bank Manufacturing
OUR TECHNOLOGIES
In-house state-of-the-art tech
revolutionizing your development programs.
CHOExpress®
cell hosts
Proven, Robust, High Yield
We developed a robust, high-performing cell-host for large-scale manufacturing of proteins. CHOExpress®-derived clones grow to densities of >30 million cells/mL, yield over 8 g/L, and show excellent scalability in bioreactors.
CHOExpress® has been proven to be robust and highly suitable for large scale manufacturing in bioreactors. The cell line combines perfectly with our transposon-based gene transfer system to generate highly productive clones, which provide stable productivity over 60+ generations. To help ensure the commercial viability of your product, all our cell lines are provided free of royalties.
CHOExpress®
A robust, scalable and highly
productive cell host.
FlexiCHO® medium.
A high-performance, chemically-defined medium for CHO cells. Its flexible composition enables tuning of Product Quality Attributes. Ideal for biosimilars.
HEKExpress®
cell hosts
Highest yielding HEK293 cell line
HEKExpress® is a fast-growing, productive and robust HEK293T-derived cell line. It is ideal for for large-scale manufacturing of recombinant proteins using stable expression.
Nearly a decade of work at ExcellGene has resulted in an aggregate-free single-cell suspension cell line that grows to high-densities in chemically defined media in bioreactors. This cell line is named HEKExpress® and it was found to display very high transfection efficiency. In fact, we have used HEKExpress® to reach the highest transient gene expression yield ever reported in the literature (> 1g/L). In addition, HEKExpress® has been shown to be ideal for production of gene transfer vector such as AAV and Lentiviruses at high titers.
Starting from the Master Cell Bank of the succesfull HEKExpress®, ExcellGene has recently developed HEK2Express®, a clone of HEKExpress that was selected for high productivity of AAV and favorable full/empty ratio. In addition, ExcellGene’s scientists have removed all T-antigen related DNA from the genome, making HEK2Express® truly “T-antigen free”.
Features
- Suitable for culture in large scale Bioreactors (> 500L)
- Fast growing – doubling time as low as 20 hours
- Grows to high cell densities (>15 million/mL) in chemically defined media
- Can be cloned by ExcellGene to provide clonal GMP-ready cell lines
- Resulting cell lines are highly stable (>4 months) and highly productivity (up to 9 g/L)
- Highly efficient in AAV production (> 10e15 vg/L)
- Fully characterized, cGMP master cell bank available
- Fed-batch culture protocol available to further increase process yield
HEK2Express®
Clonally derived, T-antigen-free host cell with superior AAV productivity & full/empty ratio.
FlexiHEK® medium.
A high performance chemically-defined medium for growth and production of recombinant proteins using HEK293 cells.
Request a free quote for your project today.
From DNA to product: do it right, from the start.